標題：Loligo論文：急性低溫脅迫對克氏原螯蝦呼吸代謝、抗氧化劑和代謝組學的影響
Abstract
Crayfish (Procambarus clarkii) aquaculture is threatened by abrupt temperature decreases caused by climatic phenomena, such as cold waves and seasonal fluctuations. In this study, crayfish were exposed to an abrupt temperature change from 17 °C to 7 °C for 24 h to investigate the effects of acute low-temperatures on respiratory metabolism, antioxidants, and metabolomics. The results showed that acute low-temperatures significantly reduced the activities of pyruvate kinase, lactate dehydrogenase, and succinate dehydrogenase in the gills and hemolymph, associated with decreases in anaerobic and aerobic respiratory capacities, and significant decreases in oxygen consumption, ammonia excretion, and maximum metabolic rates. Antioxidant enzymes in the hepatopancreas and hemolymph initially increased then decreased within 24 h. Metabolomics revealed that glycerophospholipid metabolism and glycosylphosphatidylinositol anchor biosynthesis pathways responded to acute low-temperatures, with glycerophospholipids being the most significantly differentially expressed metabolites. These results supported the hypothesis that crayfish exhibit lower metabolic activity at low temperatures. Our data provide mechanistic insight into the biological changes induced by acute low-temperature and may provide insight into culture of P. clarkii in cold waters.
摘要：
克氏原螯蝦（Procambarus clarkii）的養(yǎng)殖受到寒潮和季節(jié)波動等氣候現(xiàn)象引起的氣溫驟降的威脅。在這項研究中，小龍蝦暴露在17°C至7°C的突然溫度變化中24小時，以研究急性低溫對呼吸代謝、抗氧化劑和代謝組學的影響。結果表明，急性低溫顯著降低了鰓和血淋巴中丙酮酸激酶、乳酸脫氫酶和琥珀酸脫氫酶的活性，這與無氧和有氧呼吸能力的降低以及耗氧量、氨排泄量和最大代謝率的顯著降低有關。肝胰腺和血淋巴中的抗氧化酶在24小時內(nèi)最初增加然后減少。代謝組學顯示，甘油磷脂代謝和糖基磷脂酰肌醇錨定生物合成途徑對急性低溫有反應，其中甘油磷脂是表達差異最大的代謝物。這些結果支持了小龍蝦在低溫下表現(xiàn)出較低代謝活性的假設。我們的數(shù)據(jù)為急性低溫引起的生物變化提供了機理上的見解，并可能為克拉克氏瘧原蟲在冷水中的培養(yǎng)提供見解。
關鍵詞：呼吸代謝 Respirometry，Loligo 呼吸代謝，抗氧化劑，代謝組學
